David Wong 
Salivary Diagnostics 

Salivary Diagnostics surveys one of the most exciting areas of research in oral biology. Regarded as the mirror of the body, saliva has immense potential to yield real clinical improvements in our ability to diagnose, and hence treat, oral and systemic conditions. The composition of saliva and other oral fluids reflects the tissue fluid levels of therapeutic, hormonal, and immunological molecules, as well as the presence of markers for systemic and oral disease.

Table of Content

Contributors viii

Foreword xv

Preface xvii

Acknowledgment xviii

I Background and Foundation

1 Salivary Gland Development and Regeneration 3

Sarah M. Knox and Matthew P. Hoffman

2 Salivary Gland Physiology Relevant to Diagnostics 14

Victor G. Romanenko and James E. Melvin

3 Saliva: Properties and Functions 27

Arie van Nieuw Amerongen, Enno C.I. Veerman, and Arjan
Vissink

4 Saliva Collectors 37

Arjan Vissink, Andy Wolff, and Enno C.I. Veerman

5 Salivary Secretion in Health and Disease 60

Michael D. Turner and Jonathan A. Ship

6 Processing and Storage of Saliva Samples 69

Enno C.I. Veerman, Arjan Vissink, David T. Wong, and Arie van
Nieuw Amerongen

II Saliva Diagnostics

7 Historical Perspectives and Present 79

Frank G. Oppenheim

8 Diagnostics Other Than Blood 94

Sudhir Srivastava and Karl Krueger

9 Perceptions of Saliva: Relevance to Clinical Diagnostics
104

Sreenivas Koka and David T. Wong

10 Saliva-Based Diagnostic Technologies: Highlights of the
NIDCR’s Program 111

Eleni Kousvelari, John T. Mc Devitt, Daniel Malamud, David T.
Wong, and David R. Walt

11 Human Salivary Proteomics 122

Shen Hu, Markus Hardt, Susan J. Fisher, David T. Wong, John R.
Yates, James E. Melvin, and Joseph A. Loo

12 Genomic Targets in Saliva 129

Bernhard G. Zimmermann, Zhanzhi Hu, and David T. Wong

13 Point-of-Care Diagnostics for Infectious diseases 136

Paul L.A.M. Corstjens, Daniel Malamud

14 Dental Caries Risk Assessment 150

Paul C. Denny and Patricia A. Denny

15 Periodontal Disease 156

Christoph A. Ramseier, Thiago Morelli, Janet S. Kinney, Meghan
Dubois, Lindsay Rayburn, and William Giannobile

16 Role of Saliva in Detection of Substance Abuse 169

Uttam Garg and Lance Presley

17 Head and Neck Cancer 180

Joseph A. Califano and Suhail K. Mithani

18 Sjogren’s Syndrome 189

Philip C. Fox

19 Salivary Gland Dysfunction Associated with Systemic Disease
198

Nelson L. Rhodus

20 Progression and Treatment Evaluation in Diseases Affecting
Salivary Glands 214

Jiska M. Meijer, Cees G.M. Kallenberg, and Arjan Vissink

21 The Uses of Saliva in Forensic Genetics 226

Jack Ballantyne and Jane Juusola

22 SPKB–Salivary Proteome Knowledge Base: A Platform for
Collaborative Proteomics Research 233

Weihong Yan, Weixia Yu, Shawn Than, Renli Qiao, D. Stott Parker,
Joseph A. Loo, and David T. Wong

III Saliva Diagnostics–A New Industry

23 Commercialization of Oral Fluid Products and Technologies
243

R. Sam Niedbala

24 Coverage and Reimbursement for Salivary Diagnostic Tests
255

James J. Crall

25 Saliva Diagnostics in the Dentist’s and Physician’s Office
263

Chakwan Siew and Milton V. Marshall

26 Salivary Hormones in Research and Diagnostics 270

Douglas A. Granger, Christine K. Fortunato, Leah C.
Hibel

27 Crossroads Between Saliva Diagnostics and Salivary Gland Gene
Therapy 278

Bruce J. Baum, Ana P. Cotrim, Corinne M. Goldsmith, Fumi
Mineshiba, Senrong Qi, Gabor Z. Racz, Yuval Samuni, Takayuki
Sugito, Antonis Voutetakis, and Changyu Zheng

28 Saliva Diagnostics–A New Industry 288

Stuart R. Smith, David T. Wong, and R. Michael Buch

Index 295

About the author

David Wong is Associate Dean of Research, Professor of Oral Biology, Oral Biology & Medicine, and Director of Dental Research Institute at UCLA. Dr. Wong is a dentist-scientist focusing his research on the molecular determinants (genomic and proteomic) of head and neck cancer. His research has been continuously funded by the NIDCR and NCI for the past 18 years. He has achieved two 4-year award U01 awards from the NIDCR for the creation of the UCLA Collaborative Oral Fluid Diagnostic Research Center and the Human Salivary Proteome project.