Elmar Heinzle & Arno P. Biwer 
Development of Sustainable Bioprocesses 
Modeling and Assessment

Bioprocess technology involves the combination of living matter
(whole organism or enzymes ) with nutrients under laboratory
conditions to make a desired product within the pharmaceutical,
food, cosmetics, biotechnology, fine chemicals and bulk chemicals
sectors. Industry is under increasing pressure to develop new
processes that are both environmentally friendly and
cost-effective, and this can be achieved by taking a fresh look at
process development; – namely by combining modern process modeling
techniques with sustainability assessment methods.

Development of Sustainable Bioprocesses: Modeling and
Assessment describes methodologies and supporting case
studies for the evolution and implementation of sustainable
bioprocesses. Practical and industry-focused, the book begins with
an introduction to the bioprocess industries and development
procedures. Bioprocesses and bioproducts are then introduced,
together with a description of the unit operations involved.
Modeling procedures, a key feature of the book, are covered in
chapter 3 prior to an overview of the key sustainability assessment
methods in use (environmental, economic and societal). The second
part of the book is devoted to case studies, which cover the
development of bioprocesses in the pharmaceutical, food, fine
chemicals, cosmetics and bulk chemicals industries. Some selected
case studies include: citric acid, biopolymers, antibiotics,
biopharmaceuticals.

Supplementary material provides hands-on materials so that the
techniques can be put into practice. These materials include a demo
version of Super Pro Designer software (used in process engineering)
and models of all featured case studies, excel sheets of assessment
methods, Monte Carlo simulations and exercises.

Previously available on CD-ROM, the supplementary material can
now be accessed via href=’http://booksupport.wiley.com’>http://booksupport.wiley.com
by entering the author name, book title or isbn and clicking on the
desired entry. This will then give a listing of all the content
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downloading material.

Table of Content

Preface.

Acknowledgements.

List of Contributors.

Part I: Theoretical Introduction.

1. Introduction.

1.1. Bioprocesses.

1.2. Modeling and Assessment in Process Development.

2. Development of Bioprocesses.

2.1. Types of Bioprocesses and Bioproducts.

2.2. Bioreaction Stoichiometry, Thermodynamics and Kinetics.

2.3. Elements of Bioprocesses (Unit Operations and Unit Processes).

2.4. The Development Process.

3. Modeling and Simulation of Bioprocesses.

3.1. Problem Structuring, Process Analysis and Process Scheme.

3.2. Implementation and Simulation.

3.3. Uncertainty Analysis.

4. Sustainability Assessment.

4.1. Sustainability.

4.2. Economic Assessment.

4.3. Environmental Assessment.

4.4. Assessing Social Aspects.

4.5. Interactions Between the Sustainability Dimensions.

Part II: Bioprocess Case Studies.

Introduction to Case Studies

5. Citric Acid – Alternative Process Using Starch.

5.1. Introduction.

5.2. Fermentation Model.

5.3. Process Model.

5.4. Inventory Analysis.

5.5. Environmental Assessment.

5.6. Economic Assessment.

5.7. Conclusions.

6. Pyruvic Acid – Fermentation with Alternative Downstream Processes.

6.1. Introduction.

6.2. Fermentation Model.

6.3. Process Model.

6.4. Inventory Analysis.

6.5. Environmental Assessment.

6.6. Economic Assessment.

6.7. Conclusions.

7. L-Lysine – Coupling of Bioreaction and Process Model.

7.1. Introduction.

7.2. Basic Strategy.

7.3. Bioreaction Model.

7.4. Process Model.

7.5. Coupling of Bioreaction and Process Model.

7.6. Results and Discussion.

8. Riboflavin – Vitamin B2.

8.1. Introduction.

8.2. Biosynthesis and Fermentation.

8.3. Production Process and Process Model.

8.4. Inventory Analysis.

8.5 Ecological Assessment.

8.6 Economic Assessment.

8.7 Discussion and Concluding Remarks.

9. alpha-Cyclodextrin.

9.1. Introduction.

9.2. Reaction Model.

9.3. Process Model.

9.4. Inventory Analysis.

9.5. Environmental Assessment.

9.6. Economic Assessment.

9.7. Conclusions.

10. Penicillin V.

10.1. Introduction.

10.2. Modeling Base Case.

10.3. Inventory Analysis.

10.4. Environmental Assessment.

10.5. Economic Assessment.

10.6. Monte Carlo Simulations.

10.7. Conclusions.

11. Recombinant Human Serum Albumin.

11.1. Introduction.

11.2. Bioreaction Model.

11.3. Process Model.

11.4. Economic Assessment.

11.5. Ecological Assessment.

11.6. Conclusions.

12. Recombinant Human Insulin.

12.1. Introduction.

12.2. Market Analysis and Design Basis.

12.3. Economic Assessment.

12.4. Throughput Increase Options.

12.5. Conclusions.

13. Monoclonal Antibodies.

13.1. Introduction.

13.2. Process Model.

13.3. Inventory Analysis.

13.4. Economic Assessment.

13.5. Environmental Assessment.

13.6. Uncertainty Analysis.

13.6. Conclusions.

14. alpha-1-Antitrypsin from Transgenic Plant Cell Suspension Cultures.

14.1. Introduction.

14.2. Process Description.

14.3. Model Description.

14.4. Discussion.

14.5 Conclusions.

15. Plasmid DNA.

15.1. Introduction.

15.2. Model Description.

15.3. Inventory Analysis.

15.4. Economic Assessment.

15.5. Environmental Assessment.

15.6. Discussion.

15.7. Conclusions.

Index.

About the author

Professor Elmar Heinzle, Universität des Saarlandes,
Germany

Dr. Charles Cooney, MIT, USA

Both internationally-recognised experts in biochemical
engineering and modeling

Dr Arno Biwer, MIT/ Universität des Saarlandes,
Germany