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Over the last decade, high-sensitivity calorimetry has developed
from a specialist method used mainly by dedicated experts to a
major, commercially available tool in the arsenal directed at
understanding molecular interactions and stability. Calorimeters
have now become commonplace in bioscience laboratories. As a
result, the number of those proficient in experimentation in this
field has risen dramatically, as has the range of experiments to
which these methods have been applied. Applications extend from
studies in small molecule and solvent biophysics, through drug
screening to whole cell assays. The technology has developed to
include higher levels of sensitivity (and hence smaller sample size
requirements) and a drive towards high-throughput technology,
creating a very large user base in both academia and the
pharmaceutical industry.
This book is a fully revised and updated edition of the
successful Biocalorimetry: Applications of Calorimetry in
the Biological Sciences, published in 1998. Since then, there
have been many advances in the instrumentation as well as in its
applications and methodology. There are general chapters
highlighting the usage of the isothermal titration calorimeter and
the differential scanning calorimeter, more advanced chapters on
specific applications and tutorials that cover the idiosyncrasies
of experimental methods and data analysis. The book draws these
together to create the definitive biological calorimetric text
book.
This book both explains the background to the method and
describes novel, high-impact applications. It features works
of interest to the experienced calorimetrist and the enthusiastic
dilettante. The book should be of interest to all working in
the field of biocalorimetry, from graduate students to researchers
in academia and in industry.
from a specialist method used mainly by dedicated experts to a
major, commercially available tool in the arsenal directed at
understanding molecular interactions and stability. Calorimeters
have now become commonplace in bioscience laboratories. As a
result, the number of those proficient in experimentation in this
field has risen dramatically, as has the range of experiments to
which these methods have been applied. Applications extend from
studies in small molecule and solvent biophysics, through drug
screening to whole cell assays. The technology has developed to
include higher levels of sensitivity (and hence smaller sample size
requirements) and a drive towards high-throughput technology,
creating a very large user base in both academia and the
pharmaceutical industry.
This book is a fully revised and updated edition of the
successful Biocalorimetry: Applications of Calorimetry in
the Biological Sciences, published in 1998. Since then, there
have been many advances in the instrumentation as well as in its
applications and methodology. There are general chapters
highlighting the usage of the isothermal titration calorimeter and
the differential scanning calorimeter, more advanced chapters on
specific applications and tutorials that cover the idiosyncrasies
of experimental methods and data analysis. The book draws these
together to create the definitive biological calorimetric text
book.
This book both explains the background to the method and
describes novel, high-impact applications. It features works
of interest to the experienced calorimetrist and the enthusiastic
dilettante. The book should be of interest to all working in
the field of biocalorimetry, from graduate students to researchers
in academia and in industry.
€152.99
Table of Content
Preface.List of Contributors.
PART I: GENERAL INTRODUCTION.
1. Applications of Biocalorimetry: Binding, Stability and Enzyme
Kinetics (R. O’Brien and I. Haq).
PART II: ISOTHERMAL TITRATION CALORIMETRY.
2. Isothermal Titration Calorimetry: A Tutorial (J.A. Thomson
and J.E. Ladbury).
3. The Application of Isothermal Titration Calorimetry to Drug
Discovery (G. Holdgate. S. Fisher and W. Ward).
4. Dissecting the Thermodynamics of DNA-Protein Interactions (T.
Härd).
5. Salt Effects in Ribonuclease-Ligand Interactions: Screening
or Competitive Binding? (K.P. Murphy, T.T. Waldron and G.L.
Schrift)
6. Thermodynamic-Structure Correlations of Sulfonamide Inhibitor
Binding to Carbonic Anhydrase (D. Matulis and M. Todd).
7. Energetics of the Interaction of Human Acidic Fibroblast
Growth Factor with Heparin and the Functional Analog
Myo-inositol Hexasulfate (M. Guzmán-Casado, M.M.
Garcia-Mira, P. Cano-Soldado, J.M. Sanchez-Ruiz and A.
Parody-Morreale).
8. Thermodynamics of SH2 Domain Binding (G. Waksman, S. Kumaran
and P. Lubman).
9. Titration Calorimetry as a Tool to Determining Thermodyanmic
and Kinetic Parameters of Enzymes (M.L. Bianconi).
PART III: DIFFERENTIAL SCANNING CALORIMETRY.
10. Energetics of Site-Specific DNA Recognition by Integrase
Tn916 (S. Milev, H.R. Bosshard and I. Jelesarov).
11. Linkage Between Temperature and Chemical Denaturant Effects
on Protein Stability: The Intepretation of
Calorimetrically-Determined m Values (B. Ibarra-Malero,
R. Perez-Jimenez, R. Godoy-Ruiz and J.M. Sanchez-Ruiz).
12. Thermodymic Indications of the Molten Globule State of
Cytochrome c Induced by Hydrophobic Salts (A.A.
Moosavi-Movahedi and J. Chamani).
13. Microcalorimetry as Applied to Psychrophilic Enzymes (S.
D’Amico, D. Georlette, T. Collins, G. Feller and C.
Gerday).
14. An Autosampling Differential Scanning Calorimeter for Study
of Biomolecular Interactions (V. Plotnikov, A. Rochalski, M.
Brandts, J.F. Brandts, S. Williston, V. Frasca and L.-N. Lin).
Index.
About the author
John Edward Simon Durham LadburyWelcome Trust Senior Research Fellow/Honorary Senior Lecturer,
Department of Biochemistry & Molecular Biology, University
College London
Language English ● Format PDF ● Pages 276 ● ISBN 9780470011119 ● File size 3.0 MB ● Editor John E. Ladbury & Michael L. Doyle ● Publisher John Wiley & Sons ● Published 2005 ● Edition 2 ● Downloadable 24 months ● Currency EUR ● ID 2312187 ● Copy protection Adobe DRM
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