Richard B. Cole 
Electrospray and MALDI Mass Spectrometry 
Fundamentals, Instrumentation, Practicalities, and Biological Applications

Discover how advances in mass spectrometry are fueling new
discoveries across a broad range of research areas

Electrospray and MALDI Mass Spectrometry brings both veteran
practitioners and beginning scientists up to date with the most
recent trends and findings in electrospray ionization and
matrix-assisted laser desorption/ionization (MALDI) mass
spectrometry. In particular, this Second Edition highlights how
advances in electrospray and MALDI mass spectrometry are supporting
important discoveries in new and emerging fields such as proteomics
and metabolomics as well as in traditional areas of chemistry and
physics research.

Electrospray AND MALDI Mass Spectrometry, SECOND EDITION is
divided into five parts:

* Part A, Fundamentals of ES, explains the fundamental phenomena
underlying the electrospray process, including selectivity in
ionization and inherent electrochemistry, and concludes with a
chapter offering a comparative inventory of source hardware

* Part B, Fundamentals of MALDI, confronts ionization mechanisms,
instrument development, and matrix selection, and includes a final
chapter that explores the special application of MALDI to obtain
two-dimensional images of spatial distributions of compounds on
surfaces

* Part C, ES and MALDI Coupling to Mass Spectrometry
Instrumentation, examines the coupling of these ionization
techniques to various mass analyzers, including quadrupole ion
trap, time-of-flight, Fourier transform ion cyclotron resonance,
and ion mobility mass spectrometers

* Part D, Practical Aspects of ES and MALDI, investigates
analytical issues including quantification, charge-state
distributions, noncovalent interactions in solution that are
preserved as gas-phase ions, and various means of ion excitation in
preparation for tandem mass spectrometry, and offers a guide to the
interpretation of even-electron mass spectra

* Part E, Biological Applications of ES and MALDI, examines the
role of mass spectrometry in such areas as peptide and protein
characterization, carbohydrate analysis, lipid analysis, and drug
discovery

Written by a team of leading experts, the book not only provides
a critical review of the literature, but also presents key concepts
in tutorial fashion to help readers take full advantage of the
latest technological breakthroughs and applications. As a result,
Electrospray and MALDI Mass Spectrometry will help researchers
fully leverage the power of electrospray and MALDI mass
spectrometry. The judicious compartmentalization of chapters, and
the pedagogic presentation style throughout, render the book highly
suitable for use as a text for graduate-level courses in advanced
mass spectrometry.

Table of Content

Foreword: Desorption Ionization and Spray Ionization: Connections
and Progress (R. Graham Cooks).

Foreword to the First Edition (John B. Fenn).

Preface (Richard B. Cole).

Contributors.

Part I Fundamentals of ES.

1. On the Mechanism of Electrospray Ionization Mass Spectrometry
(ESIMS) (Paul Kebarle and Udo H. Verkerk).

2. Selectivity in Electrospray Ionization Mass Spectrometry
(Nadja B. Cech and Christie G. Enke).

3. Electrochemistry of the Electrospray Ion Source (Gary J.
Van Berkel and Vilmos Kertesz).

4. ESI Source Design (Andries P. Bruins).

Part II Fundamentals of MALDI.

5. MALDI Ionization Mechanisms: an Overview (Richard
Knochenmuss).

6. The Development of Matrix-Assisted Laser
Desorption/Ionization Sources (Peter B. O’Connor).

7. A Comparison of MALDI Matrices (Mahmud Hossain and Patrick
A. Limbach).

8. MALDI Imaging Mass Spectrometry (Alain Brunelle and
Olivier Laprévote).

Part III ES and MALDI Coupling to Mass Spectrometry
Instrumentation.

9. Coupling ESI and MALDI Sources to the Quadrupole Mass Filter,
Quadrupole Ion Trap, Linear Quadrupole Ion Trap, and Orbitrap Mass
Analyzers (Mark E. Bier).

10. Time-of-Flight Mass Spectrometry (Robert J.
Cotter).

11. Fourier Transform Ion Cyclotron Resonance and Magnetic
Sector Analyzers for ESI and MALDI (Jeremy J. Wolff and I.
Jonathan Amster).

12. Ion Mobility-Mass Spectrometry (John A. Mc Lean, J.
Albert Schultz, and Amina S. Woods).

Part IV Practical Aspects of ES and MALDI.

13. ESI, APCI, and MALDI A Comparison of the Central Analytical
Figures of Merit: Sensitivity, Reproducibility, and Speed
(Thomas R. Covey, Bradley B. Schneider, Hassan Javaheri, J.C.
Yves Le Blanc, Gordana Ivosev, Jay J. Corr, and Peter
Kovarik).

14. Charge State Distributions in Electrospray and MALDI (Yan
Li and Richard B. Cole).

15. Probing Noncovalent Interactions by Electrospray Ionization
and Matrix-Assisted Laser Desorption/Ionization (Sonal Mathur,
Alexis Nazabal, and Renato Zenobi).

16. Ion Activation Methods for Tandem Mass Spectrometry
(Kristina H& #229;akansson and John S.
Klassen).

17. Dissociation of Even-Electron Ions (Carlos Afonso,
Richard B. Cole, and Jean-Claude Tabet).

Part V Biological Applications of ES and MALDI.

18. The Role of Mass Spectrometry for Peptide, Protein, and
Proteome Characterization (A. Jimmy Ytterberg, Jason Dunsmore,
Shirley H. Lomeli, Mario Thevis, Yongming Xie, Rachel R. Ogorzalek
Loo, and Joseph A. Loo).

19. Carbohydrate Analysis by ESI and MALDI (David J.
Harvey).

20. Applications of ESI and MALDI to Lipid Analysis (Xianlin
Han).

21. In Vitro ADME Profiling and Pharmacokinetic Screening in
Drug Discovery (Daniel B. Kassel).

Index.

About the author

RICHARD B. COLE, PHD, is a University Research Professor in The Department of Chemistry at The University of New Orleans. He has spent more than twenty-five years working in the field of mass spectrometry. His research interests include analytical/bio-organic chemistry as well as fundamentals and applications of mass spectrometry, with a focus on the development of novel analytical methods employing electrospray and MALDI.