Ángel García-Alonso & Yotis Senis 
Platelet Proteomics 
Principles, Analysis, and Applications

The purpose of the book is to introduce platelets, and their
functional role in thrombotic and cardiovascular disease,
justifying the relevance of platelet proteomics research. Focus
then shifts to the recent developments on mass spectrometry
(MS)-based proteomics. This chapter shows potential
applications for platelet proteomics not yet carried out. It
includes examples of post-translational modifications (PTMs)
analysis in platelets.

The second part of the book focuses on the main research done so
far on platelet proteomics. This includes general proteome mapping
by non-gel based separation methods (Mud Pit), analysis of the
general platelet proteome and signaling cascades by gel-based
separation methods (2-DE), sub-proteome analyses
(secretome/releasate, membrane proteins, organelles). Finally, the
last section links the platelet transcriptome and application to
disease. This section is highly relevant and includes
chapters on proteomics, transcriptomics, functional genomics,
systems biology, and their applications to platelet-related
diseases.

Table of Content

Foreword xiii

Preface xv

Acronyms xvii

Part I General Overview: Platelets, Sample Preparation, and Mass Spectrometry-Based Proteomics

1 Platelets and Their Role in Thrombotic and Cardiovascular Disease: The Impact of Proteomic Analysis 3
Ronald G. Stanley, Katherine L. Tucker, Natasha E. Barrett, and Jonathan M. Gibbins

2 Mass-Spectrometry-Based Proteomics: General Overview and Posttranslational Modification Analysis in the Context of Platelet Research 27
Julia Maria Burkhart and Albert Sickmann

3 Sample Preparation Variables in Platelet Proteomics for Biomarker Research 67
Maria Zellner and Rudolf Oehler

Part II Analysis of The Platelet Proteome: Global Approaches and Subproteomes

4 Two-Dimensional Gel Electrophoresis: Basic Principles and Application to Platelet Signaling Studies 89
Ángel García

5 The Platelet Membrane Proteome 111
Yotis A. Senis

6 Proteomics of Platelet Granules, Organelles, and Releasate 139
James P. Mc Redmond

7 The Platelet Microparticle Proteome 159
David M. Smalley

8 N-Terminal Combined Fractional Diagonal Chromatographic (COFRADIC) Analysis of the Human Platelet Proteome 185
Francis Impens, Kenny Helsens, Niklaas Colaert, Lennart Martens, Joël Vandekerckhove, and Kris Gevaert

Part III Integrated ‘Omics’ and Application to Disease

9 Serial Analysis of Gene Expression (SAGE) For Studying the Platelet and Megakaryocyte Transcriptome 209
Michael G. Tomlinson

10 The Application of Microarray Analysis and Its Integration with Proteomics for Study of Platelet-Associated Disorders 231
Dmitri V. Gnatenko and Wadie F. Bahou

11 Platelet Functional Genomics 253
Isabelle I. Salles, Marie N. O’Connor, Daphne C. Thijssen-Timmer, Katleen Broos, and Hans Deckmyn

12 Systems Biology to Study Platelet-Related Bleeding Disorders 285
Jan-Willem N. Akkerman and Bernard De Bono

13 Platelet Proteomics in Transfusion Medicine 321
Thomas Thiele, Leif Steil, Uwe Völker, and Andreas Greinacher

14 Cardiovascular Proteomics 341
Fernando Vivanco, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, and Gloria Álvarez-Llamas

Index 381

About the author

DR. ÁNGEL GARCÍA is currently a Ramón y Cajal Research Fellow at the Pharmacology Department, Universidade de Santiago de Compostela, Spain. His main research focus is in the area of platelet proteomics, with special emphasis in cardiovascular research and platelet signaling studies. He has written over twenty publications in peer-reviewed journals and has been an invited lecturer at the Platelets 2010 International Symposium (Jerusalem, Israel), and the XXII Congress of the International Society on Thrombosis and Haemostasis, held in Boston, United States, in 2009, where he chaired a session on novel approaches to understanding platelet biology. Dr. García was a postdoctoral research associate of the Proteomics Group at the Oxford Glycobiology Institute, University of Oxford (UK), from 2001 to 2005.

DR. YOTIS SENIS is currently a British Heart Foundation Research Fellow at the University of Birmingham, United Kingdom. He is a senior member of the Birmingham Platelet Group. Dr. Senis’ research interests include the identification of novel platelet surface glycoproteins and determining their biological and molecular functions. He is also interested in the functional roles of protein tyrosine phosphatases in platelets and thrombosis. Dr. Senis has been an invited lecturer at the Platelet 2010 International Symposium (Jerusalem, Israel) and the Gordon Research Conference on the Cell Biology of Megakaryocytes and Platelets (Galveston, Texas). He has written over twenty publications on platelet signaling and thrombosis.