Charles Neal Stewart & Alisher Touraev 
Plant Transformation Technologies 

Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies.

With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.

Table of Content

Contributors.

Preface.

SECTION 1 Agrobacterium-Mediated
Transformation.

Chapter 1 Host Factors Involved in Genetic Transformation of
Plant Cells by

Agrobacterium (BENO^IT LACROIX, ADI ZALTSMAN,
AND VITALY CITOVSKY).

Chapter 2 Genomics of Agrobacterium-Plant
Interaction: An Approach to Refine the Plant Transformation
Technology (AJITH ANAND, ZARIR E. VAGHCHHIPAWALA, AND
KIRANKUMAR S. MYSORE).

SECTION 2 Other Transformation Technologies.

Chapter 3 Particle Bombardment: An Established Weapon in the
Arsenal of Plant Biotechnologists (THEODORE M. KLEIN).

Chapter 4 A Novel Gene Delivery System in Plants with Calcium
Alginate Micro-Beads (NAOKI WADA, SHIN’ICHIRO KAJIYAMA,
NARUEMON KHEMKLADNGOEN, AND KIICHI FUKUI).

Chapter 5 Pollen Transformation Technologies (TATIANA
RESCH AND ALISHER TOURAEV).

Chapter 6 Intragenic Vectors and Marker-Free Transformation:
Tools for a Greener Biotechnology (CAIUS M. ROMMENS, ANTHONY
CONNER, HUA YAN, AND ZAC HANLEY).

Chapter 7 Visualizing Transgene Expression (JOHN J.
FINER).

SECTION 3 Vectors, Promoters, and Other Tools for Plant
Transformation.

Chapter 8 Current State and Perspective of Binary Vectors and
Superbinary Vectors (TOSHIYUKI KOMORI AND TOSHIHIKO
KOMARI).

Chapter 9 Novel Dual Binary Vectors (p CLEAN) for Plant
Transformation (PHILIPPE VAIN).

Chapter 10 p ORE Modular Vectors for Plant Transformation
(DWAYNE D. HEGEDUS).

Chapter 11 p ANIC: A Versatile Set of Gateway-Compatible
Vectors for Gene Overexpression and RNAi-Mediated Down-Regulation
in Monocots (DAVID G.J. MANN, PETER R. LAFAYETTE, LAURA L.
ABERCROMBIE, WAYNE A. PARROTT, AND C. NEAL STEWART, JR.).

SECTION 4 Transgene Integration, Stability, Methylation,
Silencing.

Chapter 12 Understanding and Avoiding Transgene Silencing
(MICHAEL W. GRAHAM, STEPHEN R. MUDGE, PETER R. STERNES, AND
ROBERT G. BIRCH).

Chapter 13 Site-Specific Recombination for Precise and
‘Clean’ Transgene Integration in Plant Genome
(VIBHA SRIVASTAVA AND DAVID W. OW).

SECTION 5 Selection Systems, Marker-Free
Transformation.

Chapter 14 Selectable Marker Genes: Types and Interactions
(BRIAN MIKI, ASHRAF ABDEEN, YUZUKI MANABE, SOUAD EL OUAKFAOUI,
DOUGLAS A. JOHNSON, LORETA GUDYNAITE-SAVITCH, MING HU, AND PHIL
MACDONALD).

Chapter 15 Transformation Methods for Obtaining Marker-Free
Genetically Modified Plants (JAN G. SCHAART, FRANS A. KRENS,
ANNE-MARIE A. WOLTERS, AND RICHARD G.F. VISSER).

Chapter 16 Intellectual Property Aspects of Plant
Transformation (JIM M. DUNWELL).

Index.

Color Plate between pages 82 and 83.

About the author

C. Neal Stewart is Racheff Chair of Excellence in Plant
Molecular Genetics and Professor, Department of Plant Sciences,
University of Tennessee.

Alisher Touraev is Group Leader and Associated Professor
of the Institute of Microbiology and Genetics at Vienna
University.

Vitaly Citovsky is a Professor in the Department of
Biochemistry and Cell Biology at the State University of New York,
Stony Brook.

Tzvi Tzfira is Assistant Professor in Department of
Molecular, Cellular, and Developmental Biology at the University of
Michigan