Chris D. Geddes & Joseph R. Lakowicz 
Reviews in Fluorescence 2006 

This is the third volume in the Reviews in Fluorescence series. To date, two volumes have been both published and well received by the scientific community. Several book reviews have also favorably described the series as an ‘excellent compilation of material which is well balanced from authors in both the US and Europe’. Of particular mention we note the recent book review in JACS by Gary Baker, Los Alamos. In this 3rd volume we continue the tradition of publishing leading edge and timely articles from authors around the world. We hope you find this volume as useful as past volumes, which promises to be just as diverse with regard to content. Finally, in closing, we would like to thank Dr Kadir Asian for the typesetting of the entire volume and our counterparts at Springer, New York, for its timely publication. Professor Chris D. Geddes Professor Joseph R. Lakowicz August 20*^ 2005.

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The History of Fret.- Trichogin Topology and Activity in Model Membranes as Determined by Fluorescence Spectroscopy.- Theory of Metal-Fluorophore Interactions.- Current Development in the Determination of Intracellular NADH Level.- Prediction of Thermal Tissue Damage Using Fluorescence Spectroscopy.- Detection of Biological Thiols.- Fluorescent Bronchoscopy.- Quantum Dots as Fluorescent Labels for Molecular and Cellular Imaging.- Molecular Analysi S Using Microparticle-Based Flow Cytometry.- Total Internal Reflection-Fluorescence Correlation Spectroscopy.- Fluorescence Probes of Protein Dynamics and Conformations in Freely Diffusing Molecules.- Biological Application of FLIM by TCSPC.- Time-Resolved Fluorescence Anisotropy Applied to Silica Sol-Gel Growth and Surface Modification.- Dynamics of DNA and Portein-DNA Complexes Viewed Through Time-Domain Fluorescence.- Protein-Protein Interactions In Vivo: Use of Biosensors Based on FRET.- Spectroscopy for the Assessment of Melanomas.- Quantitative Fluorescence Hybridization Using Automated Image Cytometry on Interphase Nuclei.- Importance of Measuring Free Zinc in Cells.- Laser-Based Detection of Atmospheric Halocarbons.- Fret Studies of Conformational Transitions in Proteins.- Green Fluorescent Protein as a Biosensor for Toxic Compounds.- Multidimensional Fluorescence Imaging Applied to Biological Tissue.- Multiplexed Fluorescence Detection for DNA Sequencing.