This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice.
Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.
Table of Content
Preface
Acknowledgement
Contributors
Section I Current Molecular Laboratory Methods
1 Specimen Collection, Handling and Processing
Lindsy Hengesbach and John Gerlach
2 DNA/RNA Isolation and Quantitation
Jing Wang
3 Test Validation
Cindy Mc Closkey and S Terence Dunn
4 In Vitro Amplification Methods in Molecular Diagnostics
C. Alexander Valencia and Bradford Coffee
5 Whole Genome Sequencing in the Clinical Laboratory
Tina Hambuch, Brad Sickler, Arnold Liao, Suneer Jain, and Philip D. Cotter
6 Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction
Juliana Purrazzella and Arupa Ganguly
7 Reporting Clinical Molecular Genetic Laboratory Results
Victor Wei Zhang and Lora J. H. Bean
Section II Clinical Molecular Infectious Disease Testing
8 HIV-1 Genotyping and Virtual Phenotyping
Fangling Xu and Jianli Dong
9 COBAS® Ampliprep/Taqman HIV-1 Test
Tavat A. Buraruk and Charles E. Stager
10 Hepatitis C Virus (HCV) Genotype Assay (Li PA)
Charles E. Stager and Tavat A. Buraruk
11 COBAS® Ampliprep/Taqman HCV Test
Charles E. Stager and Tavat A. Buraruk
12 Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction
Su S. Chen, Seema Hai, and Rajyalakshmi Luthra
13 Quantification of BK Viral Load
Hai Wu and Jianli Dong
14 Molecular Diagnosis and Epidemiology of Rabies
Rodney E. Rohde and Bonny C. Mayes
15 Pyrosequencing for Bacterial Identification
Laura Millecker and Ruth Ann Luna
16 Other Pathogens of Significant Public Health Concern
Chris L. Mc Gowin, Rodney E. Rohde, and Greg C. Whitlock
Section III Clinical Molecular Oncology Testing
17 Gene Rearrangements Testing
Siby Sebastian, Juan Du, and Rizwan Naeem
18 Mutation Analysis in Cancer
Keyur Pravinchandra Patel, John Galbincea, and Rajyalakshmi Luthra
19 Minimal Residual Disease
Wesley Greaves and Rajyalakshmi Luthra
20 Pyrosequencing in Cancer
Bedia A. Barkoh, Rajyalakshmi Luthra, and Shalini Verma
21 Clinical Applications of Microarrays in Cancer
Marilyn M. Li, Ankita Patel, and Xiaofeng Hu
22 Basic Theories and Clinical Applications of Molecular Flow Cytometry
Hua Kang Wu, Hongli Chen, and Peter Hu
Section IV Clinical Molecular Pre/Postnatal Testing
23 Molecular Prenatal Diagnostic Testing
Joanna Wiszniewska and Christine M. Eng
24 PCR Based Diagnosis of Fragile X Syndrome
Daniel J. Dykas and Allen E. Bale
Section V Human Identity Testing
25 Microsatellite Testing Short Tandem Repeat Profile Generation
Robin De Ville Guidry and Laura Gahn
26 Parentage
Laura Gahn and Robin De Ville Guidry
Section VI Clinical Molecular Training
27 Diagnostic Molecular Training
Peter Hu, Patrick A. Lennon, and Madhuri Hegde
28 Molecular Diagnostics Point of Care Testing Training
Chang W. Lee, and Peter Hu
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