Oliver Zerbe 
BioNMR in Drug Research 

The vast progress made in the investigation of biomolecules using NMR has only recently been rewarded with the Nobel Prize for Kurt Wüthrich. Edited by a former coworker of Wüthrich, this book presents the theoretical background on NMR of biomolecules, plus the use of NMR techniques in determining the structures of proteins and nucleic acids.
Bio NMR spectroscopy offers a universal tool for examining the binding of an active substance to its target protein. Its use thereby benefits the rational development of drugs. This interaction can now be investigated in a hitherto unparalleled precision and displayed in 3D – an important prerequisite for the targeted development of new active substances. The latest methods for characterizing substance-receptor complexes are demonstrated backed by many case studies from pharmaceutical research. Thus it comes as no surprise that a large number of the authors are working for leading pharmaceutical companies.
With its successful mixture of basic information and application strategies, coupled with many real-life examples, this is an invaluable guide for both NMR spectroscopists and pharmaceutical researchers.

Table des matières

BASIC TECHNIQUES
Modern Methods for Expression of Proteins in Isotopically Enriched Form
Structure Calculation Using Automated Techniques
Achieving Better Sensitivity, Less Noise and Less Artifacts in NMR Spectra

NMR OF BIOMOLECULES
NMR Strategies for Protein Assignment
NMR of Membrane-Associated Peptides and Proteins
NMR of Nucleic Acids

MODERN SPECTROSCOPIC TECHNIQUES
Methods for the Measurement of Angle Restraints from Scalar and Dipolar Couplings as well as from Cross Correlated Relaxation: Application to Biomolecules
Orientational Restraints
Scalar Coupling Across Hydrogen Bonds
TROSY:Transverse Relaxation-Optimized Spectroscopy
MAS Solid-State NMR of Isotopically Enriched Biological Samples
Determination of Protein Dynamics Using 15N Relaxation Measurements

TOOLS FOR INVESTIGATION OF DRUG-RECEPTOR COMPLEXES AND FOR LIGAND SCREENING
The Determination of Equilibrium Dissociation Constants of Protein-Ligand Complexes by NMR
Experiments in NMR-based Screening
The Use of Spin-Labels in NMR-supported Lead Finding and Optimization
NMR of Weakly Binding Ligands
Isotope Filter and Editing Techniques

STRATEGIES FOR DRUG DEVELOPMENT USING NMR
Strategies for NMR Screening and Library Design
Strategies for Hit Finding Using NMR
Strategies for Drug Discovery Using NMR
NMR-based Drug Design: Approaches for Very Large Proteins